Introductory Chromatography Training

No of Hours: 16

SCHOOL PROGRAM/ COURSE

Introduction

  • Historical Perspective
  • Comparison of gas and liquid chromatography
  • Modern GC equipment – Packed and Capillary columns
  • Modern HPLC equipment
  • Chromatography parameters
  • Modes of liquid chromatography
  • Normal phase
  • Reverse phase

Practical & Theoretical Principles

  • Chromatographic process
  • Resolution
  • Capacity factor
  • Selectivity
  • Efficiency
  • Void volume

Chromatographic Analysis

  • Mobile phase
  • Flow rate
  • Column, pre-column and guard column
  • Column care
  • Priming and purging the pump
  • Sample preparation
  • Standard preparation
  • System suitability
  • Calculation

Gradient Elution

  • Purpose of gradient Elution
  • Gradient profiles
  • Applications
  • Practical considerations

Liquid Chro.Instrumentation

  • Pump
  • Injector
  • Column
  • Detector
  • Integrator

Gas Chro.Instrumentation

  • Injector
  • Column
  • Detector
  • Integrator

Columns

  • Column hardware
  • Pre-columns and guard columns
  • Stationary phase
  • Support
  • Particle size
  • Pore size

Normal Phase Chromatography

  • Introduction
  • Retention mechanism
  • Solvent strength
  • Problems with silica and alumina
  • Applications

Reverse Phase Chromatography

  • Basic principles
  • Bonded phase preparation
  • Surface structure
  • Retention mechanism
  • Column selection
  • Solvents
  • Solvents

Ion Exchange Chromatography

  • Separation principles in ion exchange
  • Ion exchange columns
  • Mobile phase

Size Exclusion

  • Separation principles
  • Gel Permeation vs. Gel Filtration
  • Column selection
  • Solvents
  • Calibration
  • Applications

Packed column GC

  • Support
  • Stationary phase
  • Applications

Capillary Column GC

  • Stationary phase
  • Internal diameter
  • Applications